CAS No:3326-32-7 Fluorescein isothiocyanate isomer I|FITC;FITC-CELITE(R);FITC ISOMER;FITC 'ISOMER I';FITC, ISOMER I

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Fluorescein isothiocyanate isomer I Chemical Properties

Melting point	>360 °C(lit.)

density	1.3423 (rough estimate)

refractive index	1.5060 (estimate)

storage temp.	2-8°C

solubility	Slightly soluble in DMSO, ethanol, methanol DMF and acetone

pka	2.2, 4.4, 6.7(at 25℃)

form	powder

color	White

PH Range	Weak green uorescence (6.0) to strong green uorescence (7.2)

Water Solubility	practically insoluble

Sensitive	Moisture Sensitive

λmax	490nm

BRN	1407295

Stability:	Stability Stable, but moisture-sensitive. Incompatible with strong oxidizing agents, strong bases, amines, acids.

Major Application	Magnetic nanowires, immunoassays, analyzing proteins, identifying chromosomes, Salmonella mosomes, diagnosis of cancer, kidney diseases, detecting pathogens, genes, Salmonella cells, toxoplasmosis, enzyme-mediated nucleic acid cleavage, surface molecules on colorectal cancers, treating cancer, chronic lymphocytic leukemia

InChIKey	MHMNJMPURVTYEJ-UHFFFAOYSA-N

CAS DataBase Reference	3326-32-7(CAS DataBase Reference)

EPA Substance Registry System	Spiro[isobenzofuran- 1(3H),9'-[9H]xanthen]- 3-one, 3',6'-dihydroxy- 5-isothiocyanato-(3326-32-7)



Fluorescein isothiocyanate isomer I Usage And Synthesis

Chemical Properties	Orange-yellow cryst.

Uses	Fluorescent marker for biochemical applications. Reacts under mild conditions with primary amines. Used in modification of actin at lys-61, labelling of FAB and the modification of thiol groups.

Uses	Fluorescent labeling reagent for proteins. Used in the fluorescent antibody technique for rapid identification of pathogens

Definition	ChEBI: The 5-isomer of fluorescein isothiocyanate. Acts as a fluorescent probe capable of being conjugated to tissue and proteins; used as a label in fluorescent antibody staining procedures as well as protein- and amino acid-binding techniques.

Purification Methods	It is made from the pure 5-amino isomer. Purify it by dissolving it in boiling Me2CO, filtering and adding pet ether (b 60-70o) until it becomes turbid. If an oil separates, then decant it and add more pet ether to the supernatant and cool. Orange-yellow crystals separate, collect and dry them in vacuo. It should give one spot on TLC (silica gel) in EtOAc/pyridine/AcOH (50:1:1) and in Me2NCHO/CHCl3/28% NH4OH (10:5:4). IR (Me2SO): 2110 (NCS) and 1760 (C=O) cm-1. The max 1HNMR spectra in Me2CO-d6 of the 5-and 6-isomers are distinctly different for the protons in the *benzene ring; the UV in phosphate buffer pH 8.0 shows a at ~490nm. [Sinsheimer et al. Anal Biochem 57 2271974, McKinney et al. Anal Biochem 7 74 1964, Beilstein 19 III/IV 4337.]



Fluorescein isothiocyanate isomer I Preparation Products And Raw materials

Raw materials	Etanol-->Sodium hydroxide-->Hydrochloric acid-->Ethyl acetate-->Methanol-->Dichloromethane-->Acetic acid glacial-->Toluene-->Acetic anhydride-->Palladium-->Hydrogen-->Resorcine-->Sodium hydrosulfide-->SODIUM SULPHIDE HYDRATE-->Thiophosgene-->4-Nitrophthalic acid

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